ABSTRACT
AIM:To explore the corneal biomechanical properties of the elderly with different axial length ( AL) and corneal curvature by corneal visualization Scheimpflug Technology ( Corvis ST) .?METHODS: Cross - sectional study. A total of 161 patients ( 297 eyes ) undergoing phacoemulsification were collected in this study. They were divided into 22-24mm, 24-26mm, more than 26mm groups according to axial length ( 190 eyes, 54 eyes and 53 eyes, respectively). Those of whom axial length was 22-24mm and the corneal curvature was 42-44D were divided into male and female groups ( 44 eyes and 49 eyes, respectively). Those of whom axial length was 22-24mm were divided into 42-44D group, more than 44D group according to corneal curvature ( 88 eyes, 102 eyes, respectively ) . Corvis ST was used to measure the biomechanical parameters of the cornea. The differences in the parameters between different groups were analyzed using the independent-samples t test or one-way analysis of variance and correlation analyses were performed using Pearson correlation analysis.?RESULTS:When comparing the corneal biomechanical parameters, no statistically significant differences were found between male and female groups (P>0. 05). The first applanation length and second applanation length among different corneal curvatures were statistically significant (P<0. 05). There was statistical significance on the difference of the second applanation velocity, deformation amplitude, central cornea thickness, intraocular pressure in different AL groups (P<0. 05). The axial length was positively correlated with deformation amplitude, intraocular pressure ( r=0. 429, 0. 278; P < 0. 001 ), but there was negative linear correlation between the patient's axial length and central cornea thickness, the second applanation velocity ( r=-0. 291, -0. 415;P<0. 001).?CONCLUSION: The corneal curvature and ocular axial length may be the factors affecting the corneal biomechanical characteristics. The longer axial length, the thinner corneal thickness, the more easily the corneal is deformed, and with the increase of the axial length, intraocular pressure also increases.
ABSTRACT
@#AIM:To explore the corneal biomechanical properties of the elderly with different axial length(AL)and corneal curvature by Corneal Visualization Scheimpflug Technology(Corvis ST). <p>METHODS: A cross-sectional study. A total of 220 patients(426 eyes)undergoing phacoemulsification were collected in this study. One of them whose the AL was 22-24 mm and the corneal curvature was 42-44 D were divided into male and female groups(44 eyes and 49 eyes, respectively). One of them whose the corneal curvature was 42-44 D were divided into 22-24 mm, 24-26 mm, and more than 26 mm groups according to AL(99 eyes, 22 eyes and 12 eyes, respectively). One of them whose the AL was 22-24 mm were divided into 42-44 D, and more than 44 D according to corneal curvature(88 eyes, 102 eyes, respectively). Corvis ST was used to measure the biomechanical parameters of the cornea. The differences in the parameters between different groups were analyzed using the independent-samples <i>t</i>-test or one-way analysis of variance and correlation analyses were performed using Pearson correlation analysis.<p>RESULTS: When comparing the corneal biomechanical parameters, no statistically significant differences were found between male and female groups(<i>P</i>>0.05). The first applanation length and second applanation length among different corneal curvatures were statistically significant(<i>P</i><0.05). There was statistically significant only for the difference of the second applanation length and central cornea thickness between two groups of 22-24 mm and 24-26 mm(<i>P</i><0.05). There was statistically significant for the difference of the second applanation length, deformation amplitude, central cornea thickness, the first applanation time, intraocular pressure and corrected intraocular pressure between the two groups of 22-24 mm and more than 26 mm(<i>P</i><0.05). But there was no statistically significant differences of the parameters between groups of 24-26 mm and more than 26 mm(<i>P</i>>0.05). The patient's AL was positively correlated with deformation amplitude, intraocular pressure and corrected intraocular pressure(<i>r</i>=0.263, <i>P</i>=0.002; <i>r</i>=0.463, <i>P</i>=0.000; <i>r</i>=0.449, <i>P</i>=0.000), and there is negative correlation between the patient's AL and central cornea thickness, the second applanation length(<i>r</i>=-0.240, <i>P</i>=0.006; <i>r</i>=-0.344, <i>P</i>=0.000).<p>CONCLUSION: The corneal curvature and ocular AL may be the factor affecting the corneal biomechanical properties. The longer AL, the thinner corneal thickness, the more easily the corneal is deformed, and with the increase of the AL, intraocular pressure also increases. When discussing whether the preparation of the cataract incision is affected by the patient's own factors, the different corneal curvatures and AL shall be considered.
ABSTRACT
To discuss the effect of puerarin (Pue) on the proliferation of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) and discuss whether its mechanism is achieved by regulating reactive oxygen. PASMCs of primarily cultured rats (2-5 generations) were selected in the experiment. MTT, Western blot, FCM and DCFH-DA were used to observe Pue's effect the proliferation of PASMCs. The Western blot was adopted to detect whether ROS participated in Pue's effect in inhibiting PASMC proliferation. The PASMCs were divided into five groups: the normoxia group, the hypoxia group, the hypoxia + Pue group, the hypoxia + Pue + Rotenone group and the hypoxia + Rotenone group, with Rotenone as the ROS blocker. According to the results, under the conditions of normoxia, Pue had no effect on the PASMC proliferation; But, under the conditions of hypoxia, it could inhibit the PASMC proliferation; Under the conditions of normoxia and hypoxia, Pue had no effect on the expression of the tumor necrosis factor-α (TNF-α) among PASMCs, could down-regulate the expression of hypoxia-induced cell cycle protein Cyclin A and proliferative nuclear antigen (PCNA). DCFH-DA proved Pue could reverse ROS rise caused by hypoxia. Both Rotenone and Pue could inhibit the up-regulated expressions of HIF-1α, Cyclin A, PCNA caused by anoxia, with a synergistic effect. The results suggested that Pue could inhibit the hypoxia-induced PASMC proliferation. Its mechanism may be achieved by regulating ROS.
Subject(s)
Animals , Male , Rats , Cell Cycle , Cell Proliferation , Cells, Cultured , Hypoxia , Pathology , Isoflavones , Pharmacology , Myocytes, Smooth Muscle , Physiology , Proliferating Cell Nuclear Antigen , Pulmonary Artery , Cell Biology , Rats, Wistar , Reactive Oxygen Species , MetabolismABSTRACT
To discuss the effect of puerarin (Pue) on the proliferation of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) and discuss whether the extracellular signal PI3K/AKT pathway was involved in the Pue-induced PASMC apoptosis. With the serum starvation group (SD group) as the control group, the MTT colorimetry method, Annexin V-FITC apoptosis detection kit and Western blot were used to detect Pue's effect on apoptosis of rat PASMCs. The protein immunoblot assay was used to detect whether PI3K/AKT pathway was involved in the inhibition of hypoxia-induced PASMC apoptosis process. The results show that under normoxic conditions, Pue had no effect on PASMC apoptosis; Under hypoxia conditions, Pue can inhibit PASMC apoptosis; Under normoxic and hypoxic conditions, Pue had no effect on TNF-α expression. Pue can reverse hypoxia-induced Bcl-2 (P <0.01), up-regulate it and down-regulated Bax (P <0.01). Under normoxic conditions, Pue had no effect on P-AKT expression. Both LY294002 and Pue can inhibit hypoxia-induced Bcl-2, up-regulation of P-AKT expression and down-regulation of Bax expression. Compared with the hypoxia + Pue group or the hypoxia + LY294002 group, the hypoxia + Pue + LY294002 group showed more significantly changes in Bcl-2, Bax, P-AKT expressions. The results show that, Pue can inhibit the hypoxic-induced PASMC apoptosis, which may be regulated through PI3K/AKT pathway.